Etlik Veteriner Mikrobiyoloji Dergisi
Yazarlar: Sedat ÇAM
Konular:Biyoloji, Mikrobiyoloji
DOI:10.35864/evmd.788440
Anahtar Kelimeler:Growth,Iron,Pathogen,Virulence,VvhA
Özet: Elevated levels of iron are strongly correlated with the growth and virulence of highly lethal human-pathogen Vibrio vulnificus. The present study examined the expression level of hemolysin/cytolysin-encoding gene (vvhA) and the growth rate of four clinical and four environmental strains of V. vulnificus under different iron concentrations (7, 10, 15, 30, and 50 μM). The expression levels of vvhA were determined according to the 2-ΔΔCT method. vvhA transcription was down-regulated as iron concentration increased. A significant difference was observed at the level of 30 and 50 μM ferric chloride concentrations compared to the lower concentrations in all the strains tested (p <0.001). Conversely, elevated iron concentration was significantly correlated with the higher growth rates of all the isolates (p <0.05). Increasing iron levels elevated the growth rate of the strains. Clinical strains appeared to be more correlated with high iron levels than environmental isolates. This study suggested that relatively increased growth rates of clinical strains of V. vulnificus under elevated iron concentrations might explain higher virulence of this pathogen in patients with iron overload from an underlying disease.