Türk Tarım ve Doğa Bilimleri Dergisi

Yazarlar: İdris BEKTAŞ

Konular:Fen

DOI:10.30910/turkjans.677316

Anahtar Kelimeler:Phosphate solubilizing bacteria,Maldi-tof ms,Organic acid,Soil bacteria

Özet: In this study, were investigated the effects of 117 phosphate solubilising bacteria isolated from 35 different plant rhizosphere soil on phosphate solubilising the cell density and the release of organic acids at the in vitro conditions. It was determined that the phosphate solubilising containing TCP ranged between 35.6 and 387.9 mg L-1 values of the isolates in the NBRIP medium. It was observed that the pH of the medium decreased from 6.8-7.0 to 4.5-5.7 in accurate ratio with the amount of phosphorus solubilising. 15 high phosphorus solubilising bacteria were selected and identified by MALDI-TOF MS identification system. Among the identified isolates as Enterobacter cloacae(2),Enterobacter cancerogenus, Enterobacter ludwigii, Enterobacter asburiae, Citrobacter koseri, Staphylococcus epidermidis, Microbacterium laevaniformans, Pseudomonas anguilliseptus, lagus spp. Microbacterium liquefaciens. The synthesis of organic acid(Fumaric acid, Lactic acid, Tartaric acid, Malic acid, Citric acid, Glasial acetic acid, Succinic acid and Maleic acid) which is the most important mechanism in phosphate solubilising of isolates, was determined by HPLC. Among the bacteria Enterobacter cancerogenus total amount of organic acid (10.758 mg ml-1) was the highest, the lowest amount of Microbacterium liquefaciens (2.964 mg ml-1) was determined. It was determined that total amount of organic acid was directly proportional to the amount of phosphorus solubilising by isolates and inversely proportional to pH. It was also determined that the isolates secrete high amounts of lactic acid (5.128-0.652 mg ml-1) as organic acid.

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