Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika

Yazarlar: Dian Puspita Anggraini, Devita Sulistiana, Dwi Kameluh Agustina, Almira Ulimaz

Konular:-

DOI:10.36312/e-saintika.v4i2.170

Anahtar Kelimeler:Bacillus firmus,Mg2+,Pectinase,Purification.

Özet: The purpose of study was to determine kinetic parameters and inhibitory effect of Mg 2+ ions on pectinase. Activity test performed at pH 4,5 temperature 55 oC for 50' with 10 mm concentrations of each ion 0-10mm. Galacturonic acid content, as product of pectin substrate hydrolysis, was used as basis for determining activity and analyzed by visible spectrophotometry. At concentrations 2 and 4 mm Mg 2+ act as an activator, but at concentrations 6, 8, and 10 mM as inhibitors. The value of K M with and without inhibitors is almost same, that is 0.3145% and 0.3105% but value of V m from both are different, that is 80,645 μg.ml -1.minute -1 and 62,112 μg.ml -1.minute -1. The conclusion that type of inhibitory pectinase with Mg 2+ is a non-competitive inhibition. The inhibition constant value (K i) is 26,84.

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