The Journal of Turkish Phytopathology

Yazarlar: Damla ERTİMURTAŞ, Hatice ÖZAKTAN

Konular:Fen

Anahtar Kelimeler:Identification,PCR,Stone fruits,Pseudomonas syringae,Bacterial disease

Özet: 15 Pseudomonas syringae (Ps) pathovars isolated from stone fruit trees such as peach, almond and cherry with bacterial cancer symptoms and kept in the Bacteriology laboratory stocks of Ege University Faculty of Agriculture, Department of Plant Protection were identified based on LOPAT (levan, oxidase, potato rot pectolytic activity, arginin dihydrolase, hypersensitive reaction on tobacco leaves), GATTa (gelatin liquefaction, aesculin hydrolysis, tyrosinase activity, utilization of tartaric acid), utilization of carbon tests classical methods. In addition, pathogenicity, syringomycin production and ice nucleation activity (INA) were used for the identification. Pseudomonas syringae pathovars were identified by PCR that cannot be diagnosed with classical tests. Molecular diagnosis of Pseudomonas syringae pv. syringae (Pss) strains were based on syringomycin synthesis (syrB) and Pseudomonas syringae pv. morsprunorum (Psm) strains were based on coronatine synthesis (cfl) were diagnosed using PCR. Also sequence analysis of 4 housekeeping genes (cts, rpoD, gapA, gyrB) were used to distinguish Pss and Psm. As a result of classical and molecular identification 9 of 15 Ps pathovars were identified as Pss.

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