Manas Journal of Agriculture Veterinary and Life Sciences

Yazarlar: Koray KIRIKÇI, Mehmet CAM

Konular:Ziraat, Sütçülük ve Hayvan Bilimleri

Anahtar Kelimeler:Of sheep,Multiple births,GDF9 (FecG1) gene,PCR-RFLP,Hhal enzyme

Özet: Of sheep, registered as a local type in the 2020 year, are bred in the districts of Of, Çaykara, Dernekpazarı, Hayrat in Trabzon, Turkey. The number of sheep raised as pure is around 1800 and 2000 heads, and twin birth rates are up to 35-40 %. In this study, in a total of 24 head Of sheep, the GDF9 (FecG1) gene polymorphism, which is related to multiple births in sheep, was investigated by the PCR-RFLP method. After the digestion of PCR products with the Hhal restriction enzyme, two alleles (A and G) and two genotypes (GA and GG) were identified. The frequencies of alleles A and G were found to be 0.46 to 0.54. The frequencies of genotypes GA and GG were found to be 0.92 to 0.09. To conclude, many heterozygous individuals in terms of the GDF9 gene in Of sheep provide an important opportunity to conduct the studies on its relationships with multiple births in this genotype.

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