Bilge International Journal of Science and Technology Research

Yazarlar: Neslihan NOHUT MAŞLAKCI

Konular:Mühendislik, Kimya

DOI:10.30516/bilgesci.806156

Anahtar Kelimeler:Biofilm,Chitosan,Electrospinning,Fiber,Quartz crystal microbalance

Özet: New biomaterials can be developed to prevent the formation of biofilms on biomaterials through growth and cellular attachment. In this way, biomaterials can gain new properties. In this study, the plasma modified-chitosan (PCh) was chemically modified with 5-ethoxy-2-methyl-benzofuran-3-carboxylic acid (E1). The structures of chemically modified PCh-E1 and PCh films were studied by X-ray photoelectron spectroscopy (XPS), Photoluminescence spectroscopy (PL), and Fourier transform infrared spectroscopy (FTIR). Electrospun nanofibers of PCh and PCh-E1 were produced at the microgram level onto the QCM electrode surface using in situ electrospinning and quartz crystal microbalance (QCM) in the presence of a support polymer polyvinyl alcohol (PVA). The morphologies and diameters of electrospun nanofibers were investigated by the Scanning electron microscopy (SEM). The average fiber diameters and standard deviations of PVA, PVA/PCh, and PVA/PCh-E1 were determined as 280.0 ± 58.9, 104.5 ± 35.9, and 99.4 ± 21.9 nm, respectively. Finer diameter PVA/PCh-E1 nanofibers were obtained from the PVA/PCh nanofibers. Antibiofilm activities against Pseudomonas aeruginosa (P. aeruginosa) of a QCM electrode surfaces coated with nanofibers were evaluated using the flow cell associated with the QCM. When QCM electrode coated with the PVA/PCh-E1 nanofibers was used, less negative frequency shift and the mass increase in (ΔF: -13709.5 Hz, Δm: 530.3 μg cm−2) was detected compared to the QCM electrode coated with PVA/PCh nanofibers (ΔF: -14552.7 Hz, Δm: 563.5 μg cm−2). QCM results showed that PVA/PCh-E1 nanofibers significantly decrease biofilm formation, due to a possible contribution of E1 compound.

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