Türk Tarım ve Doğa Bilimleri Dergisi

Yazarlar: Abdullah GÜLLER, Mustafa USTA

Konular:Fen

DOI:10.30910/turkjans.740195

Anahtar Kelimeler:CMV,WMV,RT-PCR,Capsid gene,Phylogenetic analysis

Özet: Melon plants exhibiting foliar deformations, mosaic pattern in different concentrations of green, and vein banding were found in Bingöl, Turkey, 2019. Specimens were gathered and screened by RT-PCR using capsid protein (CP) gene-specific primer sets to characterize and to ascertain the possible viral agents related to infected plants. About 657 bp and 822 bp DNA fragments were observed in the agarose gel of infected plants, confirming the presence of CMV (Cucumber mosaic cucumovirus) and WMV (Watermelon mosaic potyvirus). Two related DNA fragments from each recovered from agarose gel randomly were inserted in the proper cloning vector, then transformed into E. coli (JM109 strain), and finally sequenced by next-generation sequencing (NGS). Viral CP sequences obtained were deposited in GenBank (NCBI) with accession number MT361015 and MT361016 for CMV and with accession number MT413451 and 437295 for WMV. Sequences analysis revealed that CMV and WMV isolates showed high sequence consensus with their same species, 99.84%, and 99.88%, respectively. Further analysis disclosed that both Bingöl isolates showed the highest sequence similarity with China isolate (DQ399708). The consensus tree created from various CP sequences in different geographies clearly revealed that the two CMV isolates detected in this study are Subgroup IB, in the very close phylogenetic relations with Turkey, Thailand, India, and China's Subgroup IB isolates from diverse plant origins. Moreover, Bingöl WMV isolates exhibited an evolutionary affinity with isolates from melon and zucchini in France and isolate from a watermelon in China. This work is the first scientific evidence showing infection of CMV and WMV of melon plants in Bingöl province (Turkey), supported by high sequence homology and consensus trees.

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