Turkish Journal of Veterinary and Animal Sciences

Yazarlar: Aykut ÖZKUL

Konular:-

Anahtar Kelimeler:Canine parvovirus,Diagnosis,PCR,RFLP

Özet: In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.

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