Uluslararası Anadolu Ziraat Mühendisliği Bilimleri Dergisi
Yazarlar: Leyla AKKAYA, Gonca GÜNVER DALKILIÇ
Konular:Bahçe Bitkileri
Anahtar Kelimeler:Fuerte,Avocado,In vitro,MS,WPM
Özet: The aim of this study was to determine the effects of different media combinations and explant types on in vitro propagation of Fuerte avocado cultivar. In the research were used shoot tip and nodal segments as an explant sourcess, MS (1/2 macro), MS (3/4 macro) and WPM as nutrient medium. Some of the experiments established at different periods were lost due to intense infection development and browning due to intense secretion of phenolic substances. When the results obtained in the experiments were evaluated in general, the highest bud sprout rate (100%) was observed in the shoot tip explants cultured in all combinations of MS (1/2 macro) medium. Nodal segments produced a high amount of callus compared to shoot tip explants. The highest number of leaves/ explants (4 pieces) were obtained from MS (1/2 macro) + 1.0 and 1.5 mg/L BAP + 0.1 mg/L GAз medium.