Journal of Anatolian Environmental and Animal Sciences

Yazarlar: Fikri BALTA, Zeynep DENGİZ BALTA

Konular:-

DOI:10.35229/jaes.272222

Anahtar Kelimeler:Rainbow trout,V. anguillarum,API 20E,PCR,Slide agglutination test

Özet: The aim of this study is to determine the antibiotic profile, serotyping and identification of isolated bacteria from disease outbreaks in cultured rainbow trout juveniles transferred to seawater at 8°C floating net cages in the Eastern Black Sea Region of Turkey. The kidney and spleen of diseased rainbow trout that showed typical disease symptoms was streaked on the surface of T-TSA (added with 1.5% sodium chloride) and TCBS, and was incubated in the cooled incubator at 20±1°C for 48 hours. To identify of isolated bacteria was carried out by using slide agglutination, API 20E, PCR and conventional biochemical tests. V. anguillarum isolates were detected serotype O1, and sequenced PCR products were confirmed to be similar to V. anguillarum strain at the rate of 99% (with accession numbers: LK021130) compered in GenBank database. Results of the testing susceptibility to antibiotics showed that V. anguillarum isolates were resistant to sulphamethoxazole, ampicillin, erythromycin and oxytetracyclin, but all strains were found susceptible to oxolinic acid, enrofloxacin and florfenicol.

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