Uluslararası Tarım Araştırmalarında Yenilikçi Yaklaşımlar Dergisi

Yazarlar: Amina Leblalta, Harzallah Daoud, Cherrad Semcheddine , İbrahim Demirtaş

Konular:-

DOI:10.29329/ijiaar.2020.238.7

Anahtar Kelimeler:Antifungal activity,Mentha rotundifolia,Essential oil,Fusarium oxysporum,Molecular identification,GC/MS

Özet: The antifungal activity of Mentha rotundifolia essential oil, harvested in Setif (Algeria) was evaluated in vitro against a phytopathogenic fungi Fusarium oxysporum, causing damage on tomato. The molecular identification of the strain was based on a comparison (BLAST) of the sequences obtained against a database and was often supplemented by microscopic observations. After "SANGER" sequencing of the PCR products, the sequences were received in FASTA format. Analysis of M. rotundifolia essential oil by Gas Chromatography/Mass Spectrometry method (GC-MS) identified 14 compounds.  The 3-Cyclopenten-1-one, 2-hydroxy-3- (3-methyl-2-butenyl) - was the major constituent of this oil with a rate of about 89.09%. For this activity, we adopted the technique of direct contact on agar. F. oxysporum continued to grow on oil-free media at 1% and 0.1% (fungistatic effect); also on media with an oil concentration of 0.01%. While the explants taken from petri dish with essential oil concentration of 2; 4 and 10% did not grow (fungicidal effect). The very interesting antifungal effect of M. rotundifolia essential oil indicates the potential of this plant species as a source of natural fungicidal material. The present study revealed that this mint exhibited antifungal effect against F. oxysporum which provided a scientific basis for the use of this species as a good source of antifungal compounds. This preliminary work could provide a basis for the determination of sufficient and effective concentrations for in planta studies for the biological control of natural active substances of M. rotundifolia against fungal diseases.

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