Etlik Veteriner Mikrobiyoloji Dergisi

Yazarlar: Özgür AKGÜL, Özer AKGÜL, Naciye Yakut ÖZGÜR

Konular:Veteriner Hekimlik, Veteriner Hekimlik, Mikrobiyoloji

Anahtar Kelimeler:Leptospira spp.,Aşı,PCR

Özet: Leptospirosis is a zoonotic infectious disease with multiple organ involvement caused by Leptospira spp., a Gram-negative, non-spore-free, non-capsular and aerobic bacterium. For the diagnosis of leptospirosis; clinical/autopsy findings, microscopy, culture, animal experiments, serology and PCR (Polymerase Chain Reaction) methods are used. In this study, the presence of Leptospira spp. in urine samples of dogs vaccinated against Leptospirosis brought to pri-vate veterinary clinics in Istanbul province was determined by a real-time PCR technique which was reported to be a fast and sensitive method, and a comparative evaluation of Leptospira spp. positivity with urinary tract symptoms, age and gender were compared. Urine samples from 100 vaccinated dogs (56 females, 44 males) with and without urinary tract  symptoms were randomly included in the study. In order to determine the presence of Leptospira spp., DNA extraction and real-time PCR were performed in urine samples. Of the 100 dogs included in the study, 7 (7%) were positive for the presence of Leptospira spp.. Due to the irregular distribution of the positives determined by the groups and the number of cases may not be sufficient to affect the statistical significance; demographic data such as age, sex differences and PCR positivity of dogs with or withut symptoms were not found statistically significant. To understand the current importance of leptospirosis more clearly, to determine the most suitable method for diagnosis, to evaluate the preventive effects of vaccination; more studies including prospective and treatment options are needed.

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