Türk Tarım ve Doğa Bilimleri Dergisi

Yazarlar: Rezan A. BAYAT, Şebnem KUŞVURAN, Şebnem ELLİALTIOĞLU, A. Sülün ÜSTÜN

Konular:-

Anahtar Kelimeler:Cucurbita pepo,C. moschata,Salinity,Proline,SOD,CAT,GR,APX

Özet: Proline is known to play an important role as an osmolyte which maintains of integrity proteins and activated of antioxidative enzymes in plants subjected to NaCl stress. The stress factors are caused increasing to internal proline amount in plants. Exogenous proline application has been known to positive effect in salt tolerance. The effect of exogenous proline application on antioxidative enzymes activity was investigated in this study, by using the semi-salt-tolerant (A-19, C. moschata Poir.) and the salt-sensitive (Ç-1, C. pepo L.) pumpkin genotypes. Pumpkin seeds were germinated in vermiculite and the seedlings at two true-leaf stage were transferred to hydroponic culture (Hoagland solution). One week after transplanting the plants were subjected to four different applications (Control, 100 mM salt (NaCl), 100 mM salt (NaCl) + 5 mM proline, and 100 mM salt (NaCl) + 10 mM proline). Superoxide dismutase (SOD), catalaz (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) enzymes activity measurements and evaluations have been completed after 10 days of salt application. Antioxidant enzymes activities increased in the salt- and proline-treated plants very effectively compared to control plants. However, this effect was clearer in the A-19 salt tolerance genotype than the C-1 sensitive genotype. Exogenous proline was found useful in protecting the adverse effects of salt stress on plants and this effect may be responded with enzymatic defense systems against salt induced oxidative stress.

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