Türk Tarım ve Doğa Bilimleri Dergisi

Yazarlar: Halil TOKTAY, Refik BOZBUĞA, Mustafa İMREN, Ece KASAPOĞLU, İbrahim ELEKCİOĞLU

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Özet: The second stage juveniles (J2) of Root-knot nematode infect plant roots and feed on the inside roots as obligate parasites. After hatching, in case of inability to find plant roots, they die within a limited period. It is important to know the viability of second stage juveniles without feeding regarding their pathogenicity. In this study, the survivability of the second stage juveniles of root knot nematodes without feeding and the effect of different applications on hatching were investigated. In the first part of the study, the survival rate of Meloidogyne incognita and M. hapla second stage juveniles without feeding was determined. The survivability of M. incognita and M. hapla juveniles at differing time periods were determined by counting under the microscope. Juveniles were placed in a cold room in order to determine survival rate for 108 days at +4°C. The 50% of M. hapla lived until 71th day and 6,5% lived until 101st day in the cold room conditions. M. hapla juveniles entirely died on 108th day. The longest survivability of M. incognita juveniles was found on 66th day. In the second part of the study, the effect of tap water, hydrogen peroxide (H2O2) 3 different (1%, 2%, 3%) doses with 5 different temperatures (room temperature 16-22°C, 20°C, 25°C, 30°C, 35°C) applications were investigated on M. incognita egg mass hatching in laboratory conditions. The highest hatching rate was found from tap water at 30°C application and the least number of larvae hatching was determined on H2O2 applications.

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