Türk Tarım ve Doğa Bilimleri Dergisi

Yazarlar: Touseef HUSSAIN, Birpal SINGH, Firoz ANWAR

Konular:Fen

Anahtar Kelimeler:P. infestans,Potato seed tubers,Direct PCR,Diagnosis

Özet: The timely detection and appropriate identification of causal agents associated with disease of crop plants or seeds are considered to be the most important issue in formulating the management strategies for plant diseases. This is particularly important for plant diseases of a fungal nature, where disease-free planting materials is the only effective way to restrict the disease. Beside this, morphological discrimination requires special skill and the expertise of taxonomists or specialists and also time consuming. To simplify the detection, end-point polymerase chain reaction (PCR) assays were developed. Consensus sequences obtained from multiple alignments of target genes, RAPD based methodology was used to design the SCAR maker for rapid detection of P. infestans (amplified product 524bp). BLASTn was also used for in silico specificity. No cross reactivity was observed when primers were checked against other Phytophthora spp. The described primer sets allowed accurate identification and detection of P. infestans. All tests have multiple applications including screening of healthy planting materials, breeding programs and disease diagnosis.

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