
Türk Tarım ve Doğa Bilimleri Dergisi
Yazarlar: Atilla ÇAKIR, Gökhan SÖYLEMEZOĞLU
Konular:-
DOI:10.30910/turkjans.448391
Anahtar Kelimeler:Vitis vinifera L.,Hybridization,F1,SSR.
Özet: In the study, Karadimrit and Boğazkere grape cultivars were used as the maternal lines and 1103 P, 140 Ru American rootstocks as the paternal lines. After hybridization, the F1 genotypes obtained from the plant transformation were subjected to parental designation in selected genotypes for the purpose. In the Parental Assignment, European Union Research Project (GENRES 081), a total of 6, including VVMD27, VrZAG79, VrZAG62, VVMD5, VVS2 and VVMD7 microsatellite loci, which are now used as the minimum standard set by the whole world for the collection of grape varieties in Europe, SSR (simple sequence repeats)primer was used. The selected F1 genotypes (M1, M2, M3, M4) were compared in SSR loci whether they were parental alleles. As a result, parental alleles were found in SSR loci (M1, M2, M3, M4) in mixtures made from F1 genotypes. It is very important that the correct program has been proven to be applied in the cross-breeding study in the direction of the obtained results. Besides being one of the first studies made in this area, it takes the importance of studying a bit further. It is hoped that classical hybridization and molecular biotechnology can be used together to illuminate similar work to be done thereafter.
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