Türk Tarım ve Doğa Bilimleri Dergisi

Yazarlar: Veysel PARLAK, Orhan ERDOĞAN

Konular:-

DOI:10.30910/turkjans.448348

Anahtar Kelimeler:Rainbow trout (Oncorhycnhus mykiss),DDVP (2,2-Dichlorovinyl Dimethyl Phosphate),GH-I,IGF-I,IGF-II,Gene expression.

Özet: In this study, the effects of DDVP (2,2-Dichlorovin Dimethyl Phosphate) on the GH-I, IGF-I and IGF-II expression gene in rainbow trout (Oncorhycnhus mykiss) were investigated. During the study, the fish were exposed to 1.6 mg l-1 DDVP. The study was conducted on the samples taken from muscle tissue. Gene amplification was actualized with Real Time method by using primer and Probe of Tagman which are specific to gene parts and statistical analyses were conducted through setting the level of gene expression. As a result, in the studies on the tissue of muscle, decreases in the statistical significance of were identified in the activities of IGF-I and IGF-II which were in the treatment group. Moreover, the level of GH-I and IGF-I was positive in the control group while the level of IGF-II was negative and an increase was observed in the level of GH and a decrease was observed in the levels of IGF-I and IGF-II in the group of which chemicals were applied. It is determined that DDVP decreases the secretion of growth hormone on the tissues of muscle in trout.

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