Türk Tarım ve Doğa Bilimleri Dergisi

Yazarlar: Fatih Mehmet TOK

Konular:-

DOI:10.30910/turkjans.633614

Anahtar Kelimeler:Tomato,Corky root

Özet: Pyrenochaeta lycopersici is one of the most important fungal plant pathogen, which is known as sterile gray fungus on tomato and causes severe yield loss up to 40-70% in most of the temperate tomato growing areas. There is no effective chemical control methods of corky root of tomato caused by P. lycopersici. In this study, we examined variability of virulence and molecular types in P. lycopersici isolates from tomato in Hatay province of Turkey. In 2015, surveys were carried out in tomato growing areas of Hatay province of Turkey and infected plant samples showing wilting, yellowing and root rot symptoms were collected. After isolations on semi selective medium, a total of 17 Pyrenochaeta lycopersici isolates were collected and verified by ITS1/4 sequence. PCR process with Plyc1 and Plyc2 specific primers showed that 3 isolates were molecular Type1 and 14 isolates were Type2. To assess of virulence level of Pyrenochaeta lycopersici, a pathogenicity test was conducted with tomato seedlings by culture filtrate method. Disease symptoms were measured with a scale of 0-4 and the index were transformed to McKinney index. According to McKinney index, disease severity varied among isolates (65 to 95). There was no relationship between molecular types and virulence level but we detected slight relationship between location and virulence in this study. 

---