Türk Tarım ve Doğa Bilimleri Dergisi

Yazarlar: Abdullah GÜLLER, Mustafa USTA

Konular:-

DOI:10.30910/turkjans.633604

Anahtar Kelimeler:ZYMV (Zucchini yellow mosaic potyvirus),RT-PCR,Cloning,Characterization

Özet: Zucchini yellow mosaic potyvirus (ZYMV) belongs to family Potyviridae, which causes serious economic losses in many cucurbits from worldwide. In 2018 (September), leaf samples of pumpkin exhibiting typical viral symptoms including mosaic, blistering and wrinkling and healthy pumpkin leaf samples were collected from Bingöl and screened by Reverse- Transcription Polymerase Chain Reaction (RT-PCR) against ZYMV infection. Tested leaf samples were reacted positive resulting in an expected about 840 bp DNA fragments of partial coat protein (CP) gene of ZYMV. ZYMV-CP gene was further inserted into a pGEM-T Easy prokaryotic cloning vector and their partial nucleotide sequences and deduced amino acid were ascertained. The provided ZYMV- CP gene sequence consisted of 837 nucleotides in length coded for 279 amino acid residues of approximately 31.2 kDa. The isolate was denominated as ZYMV-Bingol and registered with MK689858 accession number in the NCBI. The sequence of ZYMV- Bingol CP gene were aligned with 21 isolates deposited in GenBank from different geographical location and its phylogenetic relationships were determined. Molecular analysis of the ZYMV CP gene sequence indicated the highest similarity with 100% Turkish isolate (JF317296) and the lowest with 91.64% of Korea isolate (AF062518), at nucleotide level. Moreover, phylogenetic analyses revealed that ZYMV Bingöl isolate is clustered with the Turkish- Adana isolate (JF317296) and Pakistan isolate (AB127936). ZYMV has been reported for the first time in the pumpkin plant from Bingöl province of Turkey by this study.

---