Biyolojik Çeşitlilik ve Koruma

Yazarlar: Bishnupriya BEHERA, Anath Bandhu DAS, Bhabatosh MİTTRA

Konular:-

Anahtar Kelimeler:Mangroves,Bruguiera parviflora,Bruguiera gymnorrhiza,Antioxidative enzyme,Sodium chloride

Özet: Mangroves possess special cellular mechanisms to cope with the salty and uncompromising environment. In order to access the role of protein and antioxidative enzymes defense system during high salt shock, two Bruguiera species B. parviflora and B. gymnorrhiza of the family Rhizophoraceae were exposed to high salt shock 500 mM NaCl for a short period of 6days. Total protein content in both the species decreased upon salt shock but the rate of degradation was more rapid in B. parviflora than in B. gymnorrhiza. SDS-PAGE protein profiling revealed that the protein having apparent molecular mass 90kDa, 49kDa, 33kDa, 23kDa,10kDa reduced very first after 4days of salt treatment of B. parviflora than in B. gymnorrhiza. The specific activities of catalase increased 2.4 times and 2.1 times in B. parviflora and B. gymnorrhiza upon exposure to high salt. Out of the four isoforms of catalase, CAT-2 activities enhanced 1.5 times and 1.2 times upon initial salt treatment for 4 days and 6 days. APX activity increased 1.3 times and 2.3 times in B. parviflora and B. gymnorrhiza under salt shock. A single isoform of APX enhanced 1.3 times and 1.7 times in B. parviflora and B. gymnorrhiza. The GPX activity increased 1.5 times and 1.3 times in leaves of salt treated seedlings of B. parviflora and B. gymnorrhiza. The ability of salt adaptability in both the species were discussed in context to their aforestation programme

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