Marine Science and Technology Bulletin

Marine Science and Technology Bulletin

Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity

Yazarlar: Muammer KIRICI, Muhammed ATAMANALP, Mahinur KIRICI, Şükrü BEYDEMİR

Cilt 9 , Sayı 2 , 2020 , Sayfalar 92 - 101

Konular:Biyokimya ve Moleküler Biyoloji

DOI:10.33714/masteb.709377

Anahtar Kelimeler:Glucose 6-phosphate dehydrogenase,Capoeta umbla,Enzyme purification,Inhibition,Metal ions,Liver

Özet: In this study, the in vitro effects of some metal ions (Ag+, Cd2+, Cu2+, Fe2+, Ni2+ and Pb2+) on freshwater fish Capoeta umbla liver and gill glucose 6-phosphate dehydrogenase (G6PD) have been investigated. For this purpose, C. umbla liver and gill G6PD enzymes were purified, with a specific activity of 31.52 and 22.83 EU/mg protein, 3353.2 and 736.5 fold in a yield of 19.28% and 23.15%, respectively. In order to control the enzyme purification SDS-PAGE showed a single band for the enzyme. In addition, in vitro effects of metal ions on enzyme activity were researched. As a result, Ag+, Cd2+, Cu2+, Ni2+ and Pb2+ inhibited fish liver G6PD; Ag+, Cd2+, Fe2+, Ni2+ and Pb2+ inhibited fish gill G6PD. Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag+. Our results also demonstrate that these metals might be dangerous at low micromolar concentrations for fish G6PD enzyme.

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@article{2020, title={Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity}, volume={9}, number={92–101}, publisher={Marine Science and Technology Bulletin}, author={Muammer KIRICI,Muhammed ATAMANALP,Mahinur KIRICI,Şükrü BEYDEMİR}, year={2020} }
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Muammer KIRICI,Muhammed ATAMANALP,Mahinur KIRICI,Şükrü BEYDEMİR. (2020). Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity (Vol. 9). Vol. 9. Marine Science and Technology Bulletin.
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Muammer KIRICI,Muhammed ATAMANALP,Mahinur KIRICI,Şükrü BEYDEMİR. Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta Umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity. no. 92–101, Marine Science and Technology Bulletin, 2020.
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