Records of Natural Products

Records of Natural Products

Development and Validation of High Performance Thin-Layer Chromatographic Method for Determination of α-Mangostin in Fruit Pericarp of Mangosteen Plant (Garcinia mangostana L.) using Ultraviolet – Visible Detection

Yazarlar: Himanshu Misra, Binit K. Dwivedi, Darshana Mehta, Bhupen K. Mehta, Dharam C. Jain

Cilt 3 , Sayı 4 , 2009 , Sayfalar 178 - 186

Konular:-

Anahtar Kelimeler:Garcinia mangostana L.,Hypericaceae,Α–Mangostin,HPTLC determination.

Özet: A simple, fast and precise quantitative high performance thin-layer chromatographic method has been developed for quantitative estimation of α-mangostin in fruit pericarp of Garcinia mangostana L. (Hypericaceae). Best solvent for extraction of a-mangostin optimized after screening with five solvents under same conditions using hot solid-liquid extraction through soxhlet apparatus. Methanol and chloroform gave highest and second highest recovery of a-mangostin, respectively. Plates were developed in chloroform-methanol in the ratio of 27-3 (v/v). Post-chromatographic derivatization performed using anisaldehyde-sulphuric acid reagent and scanned at 382 nm in ultraviolet-visible mode. The developed method was found to be linear in the range 1.0 to 5.0 mg spot-1, limits of detection and quantitation were 150 and 450 ng spot-1. The developed method was validated in terms of system suitability, specificity and robustness.

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@article{2009, title={Development and Validation of High Performance Thin-Layer Chromatographic Method for Determination of α-Mangostin in Fruit Pericarp of Mangosteen Plant (Garcinia mangostana L.) using Ultraviolet – Visible Detection}, volume={3}, number={178–186}, publisher={Records of Natural Products}, author={Himanshu Misra, Binit K. Dwivedi, Darshana Mehta, Bhupendra K. Mehta,  Dharam C. Jain}, year={2009} }
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Himanshu Misra, Binit K. Dwivedi, Darshana Mehta, Bhupendra K. Mehta,  Dharam C. Jain. (2009). Development and Validation of High Performance Thin-Layer Chromatographic Method for Determination of α-Mangostin in Fruit Pericarp of Mangosteen Plant (Garcinia mangostana L.) using Ultraviolet – Visible Detection (Vol. 3). Vol. 3. Records of Natural Products.
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Himanshu Misra, Binit K. Dwivedi, Darshana Mehta, Bhupendra K. Mehta,  Dharam C. Jain. Development and Validation of High Performance Thin-Layer Chromatographic Method for Determination of α-Mangostin in Fruit Pericarp of Mangosteen Plant (Garcinia Mangostana L.) Using Ultraviolet – Visible Detection. no. 178–186, Records of Natural Products, 2009.
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