Records of Natural Products

Yazarlar: Cinthia Costa de Lima, Kátia M. T. Caffaro, Maria Gabriella S. Araújo, Maria Lysete de A. Bastos, Lucia M. Conserva, Jamylle Nunes S. Ferro Emiliano Barreto

Konular:-

Anahtar Kelimeler:Myrciaria ferruginea ,Antibacterial activity,Cell viability,Fibroblast cells,MTT assa

Özet: This study evaluated for the first time the antibacterial activity, cell viability and migration ability on   3T3 murine fibroblast  cells of extracts and isolated compounds [lupeol (1), hexamethylcoruleoellagic acid (2) and a mixture of 1 and betulinaldehyde (3)] of Myrciaria ferruginea. In antibacterial assays extracts were susceptible only against S. aureus (MIC 500 μg/mL) and S. epidermidis (MIC ranging from 7.8 to 500 μg/mL) and compounds 1-3have shown no significant activity. In trials for c ell viability, with exception of MeOH-H 2O fraction from leaves (viable cells > 90%), both the crude extract and other fractions showed inhibition of cell growth (viable cells ≤ 80% at 15.625 and 31.25 μg/mL); while the samples from stems, with the exception of CHCl 3 fraction that showed strong cytotoxic effect at the lowest concentration tested (15.625 μg/mL), the other fractions were not cytotoxic. Compounds (1-3) inhibited cell viability in dose dependent manner (15.625 to 500 μg/mL). Mixture containing 1 and 3 showed inhibitions only in concentrations greater than 62.5 μg/mL while compound 2 decreased from the lowest concentration tested. In scratch wound assay, these compoundsnot increased the population of fibroblasts at concentrations less than 62.5 μg/mL.

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