Records of Natural Products

Yazarlar: Paulina Strugała, Sylwia Cyboran-Mikołajczyk, Dorota Wyspiańska, Anna Sokół-Łętowska, Narcyz Piórecki Janina Gabrielska

Konular:-

Anahtar Kelimeler:Cydonia oblonga Mill. ,Lipids peroxidation,Erythrocyte and phosphatidylcholine membranes,Human serum albumin,COX-1 and COX-2 enzymes,Fluorimetric study.

Özet: The study is concerned with biological activity of quince (Q) fruit extract (Cydonia oblonga Mill.) towards the phosphatidylcholine liposome and the natural lipid-protein erythrocyte membrane, as well as will explaining the 1 way of interaction of the extract with the membrane. The results showed that Q extract protects lipids against oxidation induced by AAPH compound to a similar extent in two models of membrane. Studies with probes (Laurdan and DPH) located at different depths within the membrane lipid bilayer showed that extract caused an increase of the packing order of the polar heads of lipids and a slight decrease in mobility of the acyl chains. Such results suggest that extract molecules associate with the lipid and lipid-protein membrane and can stop the propagation of free radicals within the bilayer by modifying the membrane fluidity. Furthermore, Q extract resulted in the inhibition of the activity of enzymes (cyclooxygenase-1 and cyclooxygenase-2) probably involved in inflammatory reactions in the body. The experimental results proved that extract components can bind to the main plasma protein – human serum albumin, and the quenching mechanism was suggested as static. The obtained quince–albumin binding constants show that the extract probably can be transported from the circulatory system to reach its target organ.

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